IgG2a Isotype Control Antibody

To serve as a true negative control for various procedures, this rabbit monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: assay dependent, Flow Cytometry: assay dependent, Immunofluorescence: assay dependent, Western blot: assay dependent, Immunohistochemistry (FFPE) : assay dependent

UniProt

Not Applicable

Host

Mouse

Immunogen

Recombinant full-length human IG1R protein was used as the immunogen for the IgG2a Isotype Control antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

IGG2a/6723

Applications

ELISA, FACS, IF, WB, IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Mouse IgG2a Isotype Control antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IgG2a Isotype Control antibody is available for research use only.

Storage Conditions

Aliquot the IgG2a Isotype Control antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the IgG2a Isotype Control antibody should be determined by the researcher.

Location

Assay dependent

Image Legend

IHC staining of FFPE human tonsil tissue with IgG2a Isotype Control antibody (clone IGG2a/6723) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.