HBsAg Antibody / Hepatitis B surface antigen

Hepatitis B surface antigen (HBsAg) is a glycoprotein on the surface of hepatitis B virus. After hepatitis B infection, HBsAg appears as the first viral marker. HBsAg can be detected in blood, saliva, breast milk, sweat, tears, nasopharyngeal secretions, semen and vaginal secretions of patients 2 to 6 months after infection with HBV. HBsAg antibodies are mainly used for the diagnosis of hepatitis B virus.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P03138

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human HBsAg protein was used as the immunogen for the HBsAg antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

HBsAg/7556R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the HBsAg antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HBsAg antibody is available for research use only.

Storage Conditions

Aliquot the HBsAg antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the HBsAg antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human HBV-infected liver with HBsAg antibody (clone HBsAG/7666R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.