SF-1 Antibody / Steroidogenic Factor 1 / NR5A1

Steroidogenic Factor 1 (SF-1) is considered an orphan nuclear receptor that belongs to subfamily 5. It was found to be a regulator of steroidogenic enzyme gene expression. Oxysterols are suggested as its ligands. It is expressed in all steroidogenic tissues, including the adrenal cortex, testicular Sertoli cells, and Leydig cells, ovarian theca, hypothalamus, and anterior pituitary. SF-1 plays an important role in adrenal and gonadal development.SF-1 is highly valuable marker to determine the adrenocortical origin of an adrenal mass.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q13285

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the SF-1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

SF1/8150R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SF-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SF-1 antibody is available for research use only.

Storage Conditions

Aliquot the SF-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SF-1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human testis tissue with SF-1 antibody (clone SF1/8150R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.