Tenascin Antibody / TNC

In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of as Tenascin or Tenascin C. Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

UniProt

P24821

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length protein was used as the immunogen for the Tenascin C antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

TNC/4805

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Tenascin C antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Tenascin C antibody is available for research use only.

Storage Conditions

Aliquot the Tenascin C antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Tenascin C antibody should be determined by the researcher.

Location

Connective tissue matrix

Image Legend

IHC staining of FFPE human lung tissue with Tenascin C antibody (clone TNC/4805) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.