TNFSF15 Antibody / VEGI / TL1A

VEGI is an anti-angiogenic cytokine that belongs to tumor necrosis factor superfamily, member 15 (TNFSF15) . This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Reduced expression of VEGI has been reported as a marker of poor prognosis in breast cancer.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O95150

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the TNFSF15 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

VEGI/7799R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the TNFSF15 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TNFSF15 antibody is available for research use only.

Storage Conditions

Aliquot the TNFSF15 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the TNFSF15 antibody should be determined by the researcher.

Location

Secreted, Cell Surface, Cytoplasm

Image Legend

IHC staining of FFPE human colon tissue with TNFSF15 antibody (clone VEGI /7799R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.