MART-1 Antibody / Melan-A

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

Q16655

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment corresponding to the N-terminus of human Melan A protein was used as the immunogen for the MART-1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MLANA/8108R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the MART-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MART-1 antibody is available for research use only.

Storage Conditions

Aliquot the MART-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the MART-1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Western blot testing of human COLO-38 cell lysate with recombinant MART-1 antibody (clone MLANA/8108R) . Expected molecular weight ~20 kDa with possible doublet.