Carbonic Anhydrase IX Antibody / CAIX / CA9

Carbonic Anhydrase IX catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q16790

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human protein fragment (within amino acids 314-410) was used as the immunogen for the Carbonic Anhydrase IX antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CA9/4019

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Carbonic Anhydrase IX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Carbonic Anhydrase IX antibody is available for research use only.

Storage Conditions

Aliquot the Carbonic Anhydrase IX antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Carbonic Anhydrase IX antibody should be determined by the researcher.

Location

Cell Surface, Cytoplasm

Image Legend

IHC staining of FFPE human kidney tissue with Carbonic Anhydrase IX antibody (clone CA9/4019) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.