PAX5 Antibody

The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q02548

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the PAX5 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PCRP-PAX5-1B7

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the PAX5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PAX5 antibody is available for research use only.

Storage Conditions

Aliquot the PAX5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the PAX5 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human tonsil tissue with PAX5 antibody (clone PCRP-PAX5-1B7) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.