Carbonic Anhydrase 3 Antibody / CA3

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA III (carbonic anhydrase III), also known as Car3 or CA3, is a 260 amino acid cytoplasmic protein that is specifically expressed in muscle. Belonging to the a-carbonic anhydrase family, CA III is activated by proton donors such as imidazole and dipeptide histidylhistidine, and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P07451

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of human protein was used as the immunogen for the Carbonic Anhydrase 3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CA3/7883

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Carbonic Anhydrase 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Carbonic Anhydrase 3 antibody is available for research use only.

Storage Conditions

Aliquot the Carbonic Anhydrase 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Carbonic Anhydrase 3 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human skeletal muscle tissue with Carbonic Anhydrase 3 antibody (clone CA3/7883) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.