Enhancer of zeste homolog 2 Antibody / EZH2

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q15910

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 300-550) from the human protein was used as the immunogen for the Enhancer of zeste homolog 2 antibody.

Clonality

Monoclonal

Isotype

IgG κ

Clone

EZH2/6988

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the EZH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Enhancer of zeste homolog 2 antibody is available for research use only.

Storage Conditions

Aliquot the EZH2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the EZH2 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human bladder carcinoma with Enhancer of zeste homolog 2 antibody (clone EZH2/6988) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.