Paired box 4 Antibody / PAX4

Pax-4 (paired box gene 4) protein influences normal differentiation of Insulinproducing beta cells and influences normal pancreatic islet development. Pax-4 protein is a transcriptional repressor that binds to a common cis element in the Glucagon, Insulin and Somatostatin promoters. Mouse Pax-4 transcript is present in pancreatic islets, and the islet beta cell lines MIN6, beta TC and NIT1. Differentiation of endoderm-derived endocrine pancreas is mediated through Pax-4 and Pax-6. Pax-4 may act as a Pax-6 repressor due to the competition for binding sites and lower transactivation potential of Pax-4. The human Pax-4 gene encodes a deduced 350 amino acid protein that is 80% identical to the deduced mouse Pax-4 protein.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O43316

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the Paired box 4 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PAX4/7599

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the Paired box 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Paired box 4 antibody is available for research use only.

Storage Conditions

Aliquot the Paired box 4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Paired box 4 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human ovarian carcinoma tissue with Paired box 4 antibody (clone PAX4/7599) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.