P-glycoprotein Antibody / MDR1 / ABCB1

Cells selected for resistance to a single cytotoxic drug may become crossresistant to a broad range of drugs with different structures and cellular targets. This phenomenon is called multiple drug resistance (MDR) . MDR proteins (Mdrs) are members of a highly conserved superfamily of ATP-binding cassette transport proteins. Mdr-1 is an apical transmembrane protein that is an integral part of the blood-brain barrier and functions as a drug-transport pump transporting a variety of drugs from the brain back into the blood. The Mdr-1 gene is known as ABCB1 and is located on human chromosome 7q21.12. The mouse homolog of Mdr-1 is known as Mdr-3. Interestingly, a murine protein by the name of Mdr-1 exists and is encoded by the murine Abcb1b gene, but it is not homologous with human Mdr-1.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P08183

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 500-700) from the human protein was used as the immunogen for the P-glycoprotein antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MDR1/8962R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the P-glycoprotein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This P-glycoprotein antibody is available for research use only.

Storage Conditions

Aliquot the P-glycoprotein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the P-glycoprotein antibody should be determined by the researcher.

Location

Membrane

Image Legend

IHC staining of FFPE human adrenal gland tissue with MDR1 antibody (clone MDR1/8962R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.