HSPB1 Antibody / HSP70
HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian Alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P04792
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human full-length protein was used as the immunogen for the HSPB1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RHSPB1/7298
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the HSPB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HSPB1 antibody is available for research use only.
Storage Conditions
Aliquot the HSPB1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the HSPB1 antibody should be determined by the researcher.
Location
Cytoplasm, Nucleus
Image Legend
Western blot testing of human liver and MCF7 cell lysate with HSP27 / HSPB1 antibody (clone rHSPB1/7298) . Predicted molecular weight: 23-27 kDa.
Documents
Frequently Asked Questions
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