IL-6 Antibody / Interleukin 6

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. It plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. It is involved in host defense, acute phase reactions, immune responses, and hematopoiesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P05231

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the IL-6 antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

IL6/4642

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the IL-6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IL-6 antibody is available for research use only.

Storage Conditions

Aliquot the IL-6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the IL-6 antibody should be determined by the researcher.

Location

Cytoplasm, Extracellular (secreted)

Image Legend

IHC staining of FFPE human pancreas tissue with IL-6 antibody (clone IL6/4642) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.