Epidermal Growth Factor Receptor Antibody / EGFR

This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P00533

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 300-500) from the human protein was used as the immunogen for the Epidermal Growth Factor Receptor antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

EGFR/8933R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Epidermal Growth Factor Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Epidermal Growth Factor Receptor antibody is available for research use only.

Storage Conditions

Aliquot the Epidermal Growth Factor Receptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Epidermal Growth Factor Receptor antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

Western blot testing of A431 lysate using Epidermal Growth Factor Receptor antibody. Expected molecular weight: 134-180 kDa depending on glycosylation level.