Arginase 1 Antibody / ARG1
Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1) . Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P05089
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human protein fragment (within amino acids 1-200) was used as the immunogen for the Arginase 1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
ARG1/8869R
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Arginase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Arginase 1 antibody is available for research use only.
Storage Conditions
Aliquot the Arginase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Arginase 1 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
SDS-PAGE analysis of purified, BSA-free Arginase 1 antibody (clone ARG1/8869R) as confirmation of integrity and purity.
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