HLA-ABC Antibody
Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C) . Immunoprecipitation of lactoperoxidase radioiodinated cell surface proteins and surface sialoglycoproteins radiolabeled by sodium metaperiodate/tritiated sodium borohydride technique confirmed the structure gp44, p12 (consisting of a 44kDa glycopeptide linked with a non-glycosylated 12kDa peptide) typical for MHC class I antigen (s) as a structure recognized by Bra23/9. Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P01889, P30443, P30499
Host
Mouse
Reactivity
Human
Immunogen
Human REH cells (a pre-B leukaemia cell line) was used as the immunogen for the HLA-ABC antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
Bra-23/9
Applications
FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the HLA-ABC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HLA-ABC antibody is available for research use only.
Storage Conditions
Aliquot the HLA-ABC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the HLA-ABC antibody should be determined by the researcher.
Location
Cell Surface
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