HCG-alpha Antibody

Human chorionic gonadotropin antibody (hCG) is a glycoprotein hormone synthesized in syncytiotrophoblastic cells of placenta and in certain trophoblastic tumors. The hormone-specific alpha chains have molecular weights of 13 kDa. HCG is found in moles and choriocarcinoma, chorionic components of germ cell tumors, and syncytiotrophoblast like cells in seminoma/dysgerminoma and embryonal carcinoma. In diagnostic pathology, hCG is a useful marker for classification of germ cell tumors, identification of extragonadal germ cell tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P01215

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-116) from the human protein was used as the immunogen for the HCG-alpha antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

HCGa/7875

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the HCG-alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HCG-alpha antibody is available for research use only.

Storage Conditions

Aliquot the HCG-alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the HCG-alpha antibody should be determined by the researcher.

Location

Cytoplasm, Secreted

Image Legend

IHC staining of FFPE human placental tissue with HCG-alpha antibody (clone hCGa/7875) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.