Secretagogin Antibody / SCGN

SCGN, also known as Secretagogin, CALBL, setagin or SECRET, is a 276 amino acid cytoplasmic protein that contains six EF-hand domains and is related to the calicium-binding proteins Calretinin and Calbindin D28K. Expressed in a variety of tissues including stomach, thyroid, colon, brain and neuroendocrine cells, SCGN is thought to be involved in cell proliferation and KCl (potassium chloride) -mediated calcium flux events. Through its interaction with KCl and its subsequent ability to modulate calcium storage pools within the cell, SCGN may function to negatively control growth and differentiation rates and, thus, indirectly inhibit cell replication.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O76038

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 100-276) from the human protein was used as the immunogen for the Secretagogin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SCGN/7321

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Secretagogin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Secretagogin antibody is available for research use only.

Storage Conditions

Aliquot the Secretagogin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Secretagogin antibody should be determined by the researcher.

Location

Cytoplasm, Secreted

Image Legend

IHC staining of FFPE human pancreas tissue with Secretagogin antibody (clone SCGN/7321) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.