ZSCAN12 Antibody

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kruppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Belonging to the Kruppel C2H2-type zinc-finger protein family, ZFP96 (zinc finger protein 96 homolog), also known as ZSCAN12 (zinc finger and SCAN domain-containing protein 12) and zinc finger protein 305, is a 604 amino acid nuclear protein that contains one SCAN box domain and 11 C2H2-type zinc fingers. ZFP96 is upregulated by 8-fold from day 13 of pregnancy to day 1 post-partum, suggesting that ZFP96 functions as a transcription factor by switching off pro-survival genes and/or upregulating pro-apoptotic genes of the corpus luteum.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

UniProt

O43309

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 37-132) from the human protein was used as the immunogen for the ZSCAN12 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-ZSCAN12-2B2

Applications

FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the ZSCAN12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ZSCAN12 antibody is available for research use only.

Storage Conditions

Aliquot the ZSCAN12 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ZSCAN12 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Flow cytometry testing of PFA-fixed human HeLa cells with ZSCAN12 antibody (clone PCRP-ZSCAN12-2B2) followed by goat anti-mouse IgG-CF488 (blue), Red = unstained cells.