POU5F1 Antibody / OCT4

OCT4 (Octamer-binding protein 4), also called POU5F1, is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q01860

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment of the human protein was used as the immunogen for the POU5F1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

OCT4/8098R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the POU5F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This POU5F1 antibody is available for research use only.

Storage Conditions

Aliquot the POU5F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the POU5F1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human seminoma tissue with POU5F1 antibody (clone OCT4/8098R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.