Allograft inflammatory factor 1 Antibody / AIF1 / IBA1

AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC) .

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P55008

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-146) from the human protein was used as the immunogen for the Allograft inflammatory factor 1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

AIF1/8970R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Allograft inflammatory factor 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Allograft inflammatory factor 1 antibody is available for research use only.

Storage Conditions

Aliquot the Allograft inflammatory factor 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Allograft inflammatory factor 1 antibody should be determined by the researcher.

Location

Cytoplasm, Cell Surface

Image Legend

IHC staining of FFPE human spleen tissue with Allograft inflammatory factor 1 antibody (clone AIF1/8970R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.