HSPA1A Antibody / HSP70-1A

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P0DMV8.1

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the HSPA1A antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

HSPA1A/7936

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the HSPA1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HSPA1A antibody is available for research use only.

Storage Conditions

Aliquot the HSPA1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the HSPA1A antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human kidney tissue with HSPA1A antibody (clone HSPA1A/7936) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.