BTBD9 Antibody / BTB/POZ domain-containing protein 9

BTBD9 (BTB/POZ domain-containing protein 9) is a 612 amino acid protein that contains one BTB/POZ domain and one BACK (BTB/Kelch associated) domain. The BTB/POZ domain mediates homomeric and heteromeric POZPOZ interactions and is common to transcriptional regulators involved in chromatin modeling. In several BTB/POZ containing proteins, including Bcl-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. This suggests that BTBD9 may play a role in transcription regulation. Genetic variations in the gene that encodes BTBD9 have been associated with susceptibility to restless legs syndrome type 6 (RLS6), a condition characterized by an uncontrollable urge to move the legs while resting.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q96Q07

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the BTBD9 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

BTBD9/7501

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the BTBD9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This BTBD9 antibody is available for research use only.

Storage Conditions

Aliquot the BTBD9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the BTBD9 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human fallopian tube tissue with BTBD9 antibody (clone BTBD9/7501) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.