MCM3 Antibody
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P25205
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 650-750) from the human protein was used as the immunogen for the MCM3 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MCM3/3221
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the MCM3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This MCM3 antibody is available for research use only.
Storage Conditions
Aliquot the MCM3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the MCM3 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Frequently Asked Questions
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