MCM3 Antibody

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P25205

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 650-750) from the human protein was used as the immunogen for the MCM3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MCM3/3221

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the MCM3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MCM3 antibody is available for research use only.

Storage Conditions

Aliquot the MCM3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the MCM3 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human colon carcinoma tissue with MCM3 antibody (clone MCM3/3221) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.