CD168 Antibody / HMMR

Hyaluronic acid (HA) is a nonsulfated glycosaminoglycan that regulates cell adhesion and migration. HA effects are mediated through two receptors, CD44 (also designated HCAM) and the receptor of hyaluronic acid mediated motility (RHAMM) . RHAMM, also designated intracellular hyaluronic acid binding protein (IHABP) and CD168, is a matrix receptor, which is linked to the plasma membrane by a GPI anchor and regulates cell motility. RHAMM expression is upregulated in malignant lymphoid tissues and is subsequently implicated in tumor progression and metastasis formation, as well as signal transduction. Although still unclear, RHAMM is thought to exist as several isoforms ranging in size. A variant isoform, designated v4, is a protein that when over-expressed, is thought to be the cause of transformation and metastasis formation in fibroblasts.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O75330

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-250) from the human protein was used as the immunogen for the CD168 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

HMMR/8021

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CD168 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD168 antibody is available for research use only.

Storage Conditions

Aliquot the CD168 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD168 antibody should be determined by the researcher.

Location

Cell surface, Cytoplasm

Image Legend

IHC staining of FFPE human placental tissue with CD168 / HMMR antibody (clone HMMR/8021) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.