CD74 Antibody / CLIP
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV) . CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P04233
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant fragment of human CD74 protein was used as the immunogen for the CD74 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
CLIP/8680R
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CD74 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD74 antibody is available for research use only.
Storage Conditions
Aliquot the CD74 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD74 antibody should be determined by the researcher.
Location
Cell surface, Paranuclear globular
Image Legend
Western blot testing of human Raji cell lysate with CD74 antibody (clone CLIP/8680R) . Expected molecular weight: 33-43 kDa.
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