SPARC Antibody / Osteonectin
SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P09486
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the SPARC antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
OSTN/8528R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the SPARC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SPARC antibody is available for research use only.
Storage Conditions
Aliquot the SPARC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the SPARC antibody should be determined by the researcher.
Location
Secreted
Image Legend
Documents
Frequently Asked Questions
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