OCLN Antibody / Occludin

Occludin/OCLN is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

Q16625

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human protein fragment (within amino acids 282-415) was used as the immunogen for the OCLN antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

ROCLN/8776

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the OCLN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This OCLN antibody is available for research use only.

Storage Conditions

Aliquot the OCLN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the OCLN antibody should be determined by the researcher.

Location

Cell Surface, Cytoplasm

Image Legend

IHC staining of FFPE human kidney tissue with OCLN antibody (clone rOCLN/8776) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.