OCLN Antibody / Occludin
Occludin/OCLN is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin.
Product Specifications
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
Q16625
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human protein fragment (within amino acids 282-415) was used as the immunogen for the OCLN antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2b κ
Clone
ROCLN/8776
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the OCLN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This OCLN antibody is available for research use only.
Storage Conditions
Aliquot the OCLN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/ocln-antibody-occludin-rocln8776-v5087
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the OCLN antibody should be determined by the researcher.
CAS Number
9000-83-3
Location
Cell Surface, Cytoplasm
Image Legend
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