OCLN Antibody / Occludin
Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial Occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
Q16625
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human protein fragment (within amino acids 282-415) was used as the immunogen for the OCLN antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
ROCLN/8525
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the OCLN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This OCLN antibody is available for research use only.
Storage Conditions
Aliquot the OCLN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the OCLN antibody should be determined by the researcher.
Location
Cell Surface, Cytoplasm
Image Legend
Western blot testing of human kidney tissue lysate with OCLN antibody. Predicted molecular weight ~59 kDa.
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