Occludin Antibody / OCLN
Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial Occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q16625
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human protein fragment (within amino acids 282-415) was used as the immunogen for the Occludin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
OCLN/2182
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Occludin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Occludin antibody is available for research use only.
Storage Conditions
Aliquot the Occludin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Occludin antibody should be determined by the researcher.
Location
Cell Surface, Cytoplasm
Image Legend
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