NR3C2 Antibody / Mineralocorticoid Receptor

Mineralocorticoid hormones are primarily found in epithelial tissues where they function as regulators of Na+, K+ and H+ ion transport. Aldosterone is a mineralocorticoid that has been shown to regulate electrolyte excretion and intravascular volume and is therefore involved in blood pressure regulation. Mineralocorticoid receptor (MCR or MR) is a member of the steroid/thyroid/ retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor downregulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation) . MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells

UniProt

P08235

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 601-673) from the human protein was used as the immunogen for the NR3C2 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

PCRP-NR3C2-1E3

Applications

FACS

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the NR3C2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NR3C2 antibody is available for research use only.

Storage Conditions

Aliquot the NR3C2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the NR3C2 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

Analysis of a HuProt (TM) microarray containing more than 19,000 full-length human proteins using NR3C2 antibody (PCRP-NR3C2-1E3) . Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.