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BFGF Antibody / Basic FGF / FGF2

Fibroblast growth factor-1 (FGF-1), also designated acidic FGF, and fibroblast growth factor-2 (FGF-2), also designated basic FGF, are members of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (hst/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10-FGF-23. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P09038

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 7-96) from the human protein was used as the immunogen for the bFGF antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

FGF2/7364

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the bFGF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This bFGF antibody is available for research use only.

Storage Conditions

Aliquot the bFGF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/bfgf-antibody-basic-fgf-fgf2-fgf27364-v5062

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the bFGF antibody should be determined by the researcher.

CAS Number

9007-83-4

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human placental tissue with bFGF antibody (clone FGF2/7364) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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