What sizes are available for Lymphoid enhancer-binding factor 1 Antibody / LEF1 / TCF1 alpha?

Lymphoid enhancer-binding factor 1 Antibody / LEF1 / TCF1 alpha is available in the following size(s): 20 µg.

Lymphoid enhancer-binding factor 1 Antibody / LEF1 / TCF1 alpha

Q: What is the catalog number for Lymphoid enhancer-binding factor 1 Antibody / LEF1 / TCF1 alpha?

The catalog number for Lymphoid enhancer-binding factor 1 Antibody / LEF1 / TCF1 alpha is V5057-20UG.

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells.Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q9UJU2

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-300) from the human protein was used as the immunogen for the Lymphoid enhancer-binding factor 1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

LEF1/8629R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Lymphoid enhancer-binding factor 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Lymphoid enhancer-binding factor 1 antibody is available for research use only.

Storage Conditions

Aliquot the Lymphoid enhancer-binding factor 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Lymphoid enhancer-binding factor 1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE analysis of human tonsil tissue with Lymphoid enhancer-binding factor 1 antibody (clone LEF1/8629R) . Strong nuclear staining of non-germinal center cells observed. Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.