TCF1 alpha Antibody / T cell specific transcription factor 1 alpha / LEF1

Lymphoid Enhancing Factor 1 (LEF1), also called TCF1 alpha, is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q9UJU2

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of human LEF1 protein was used as the immunogen for the TCF1 alpha antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

LEF1/6764

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the TCF1 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TCF1 alpha antibody is available for research use only.

Storage Conditions

Aliquot the TCF1 alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the TCF1 alpha antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human tonsil tissue with TCF1 alpha antibody (clone LEF1/6764) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.