Interleukin-2 Antibody / IL-2

Interleukin-2 (IL-2) is a T cell stimulatory cytokine that induces T cell proliferation and NK cell proliferation and activation. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7) . IL-2 induces CTLA-4 and also functions as a survival factor for lymphocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P60568

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-153) from the human protein was used as the immunogen for the Interleukin-2 antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

IL2/4985

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Interleukin-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Interleukin-2 antibody is available for research use only.

Storage Conditions

Aliquot the Interleukin-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Interleukin-2 antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human stomach tissue with Interleukin-2 antibody (clone IL2/4985) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.