UchL1 Antibody / PGP9.5
This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1) . Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P09936
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human UCHL1 protein was used as the immunogen for the UchL1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RUCHL1/8057
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the UchL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This UchL1 antibody is available for research use only.
Storage Conditions
Aliquot the UchL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the UchL1 antibody should be determined by the researcher.
Location
Cytoplasm, ER membrane
Image Legend
Western blot testing of human brain tissue lysate with UchL1 antibody. Predicted molecular weight ~25 kDa.
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