BCL6 Antibody / B-Cell Lymphoma 6 Protein

Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) .The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells

UniProt

P41182

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 528-601) from the human protein was used as the immunogen for the BCL6 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-BCL6-1B1

Applications

FACS

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the BCL6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This BCL6 antibody is available for research use only.

Storage Conditions

Aliquot the BCL6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the BCL6 antibody should be determined by the researcher.

Location

Nucleus, Mitochondria

Image Legend

Analysis of a HuProt (TM) microarray containing more than 19,000 full-length human proteins using BCL6 antibody (clone PCRP-BCL6-1B1) . Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.