Claudin18.2 Antibody
The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, occludin and junction adhesion molecule. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Emerging evidence suggests that the claudin family of proteins regulates transport through tight junctions via differential discrimination for solute size and charge. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P56856
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 1-100) from the human protein was used as the immunogen for the Claudin18.2 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
CLDN18.2/8559R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Claudin18.2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Claudin18.2 antibody is available for research use only.
Storage Conditions
Store the Claudin18.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Claudin18.2 antibody should be determined by the researcher.
Location
Cell junction, Cell membrane
Image Legend
Western blot testing of human pancreas tissue lysate with Claudin18.2 antibody. Predicted molecular weight ~28 kDa.
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