ALDH1L1 Antibody

Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde, lipid peroxidation and metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH1L1 (aldehyde dehydrogenase 1 family member L1), also known as FTHFD or 10-FTHFD (10-formyltetrahydrofolate dehydrogenase), is a cytosolic protein that is developmentally regulated in the cerebellum. ALDH1L1 binds to folate and catalyzes the conversion of 10-formyltetrahydrofolate (10-FTHF) to tetrahydrofolate (THF) . This suggests a possible role for ALDH1L1 in the regulation of cellular THF levels as well as in the inhibition of cell proliferation (as 10-FTHF is essential for the synthesis of purine) . In addition, the overexpression of ALDH1L1 can restrict cell proliferation in vitro.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O75891

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of human ALDH1L1 protein was used as the immunogen for the ALDH1L1 antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

ALDH1L1/7969

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the ALDH1L1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ALDH1L1 antibody is available for research use only.

Storage Conditions

Aliquot the ALDH1L1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ALDH1L1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human renal cell carcinoma tissue with ADH1L1 antibody (clone ALDH1L1/7969) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.