GCSAM Antibody / HGAL
Recognizes a protein identified as HGAL, also called GCSAM (Germinal center-associated signaling and motility protein) . It contains a putative PDZ-interacting domain, an immunoreceptor tyrosine-based activation motif (ITAM), and two putative SH2 binding sites. In B cells, its expression is specifically induced by interleukin-4. HGAL is specifically expressed in germinal center B-cells, but is absent in mantle and marginal zone B-cells and in the inter-follicular and para-cortical regions in normal tonsils and lymph nodes. Its high degree of specificity for germinal center B-cells makes anti-HGAL an ideal marker for the detection of germinal center-derived B-cell lymphomas. HGAL expression has been used to help elucidate nodal marginal zone lymphoma (NMZL) from cases of diffuse follicle center lymphoma. Additionally, HGAL expression was shown to correlate with survival in patients with diffuse large B-cell lymphoma (DLBCL) .
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q8N6F7
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the GCSAM antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
HGAL/2834
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the GCSAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This GCSAM antibody is available for research use only.
Storage Conditions
Aliquot the GCSAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the GCSAM antibody should be determined by the researcher.
Location
Cytoplasm, Cell membrane
Image Legend
Frequently Asked Questions
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