IRS1 Antibody / Insulin receptor substrate 1

The Insulin receptor substrate-1 (IRS-1), a protein major substrate of the Insulin receptor, is phosphorylated in response to stimulation of cells by Insulin, Insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4) . IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An Insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the Insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P35568

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the IRS1 antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

IRS1/7571

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the IRS1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IRS1 antibody is available for research use only.

Storage Conditions

Aliquot the IRS1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the IRS1 antibody should be determined by the researcher.

Location

Cytoplasm, Cell surface, Nucleus

Image Legend

IHC staining of FFPE human lung tissue with IRS1 antibody (clone IRS1/7571) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.