Inhibin alpha Antibody

It recognizes a 47kDa protein, which is identified as alpha sub-unit of Inhibin. It is a gonadal protein that preferentially suppresses the secretion of pituitary follicle-stimulating hormone (FSH) . Inhibin comprises two subunits, Inhibin A and Inhibin B. Each subunit consists of the same Alpha subunit, covalently linked to 1 of 2 distinct subunits, beta-Alpha or beta-beta. Originally isolated from ovarian follicular fluid and characterized as a disulphide-linked dimeric glycoprotein, inhibin belongs to the transforming growth factor beta (TFGbeta) superfamily. Antibodies against Inhibin are useful in making a differentiation between adrenal cortical tumors and renal cell carcinoma. Sex cord stromal tumors of the ovary as well as trophoblastic tumors also demonstrate cytoplasmic positivity. Inhibin antibody is also used to make the differential diagnosis of intra-uterine vs. ectopic pregnancy in endometrial curetting.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P05111

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment of human Inhibin alpha protein (within amino acids 233-362) was used as the immunogen for the Inhibin alpha antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

INHA/8872R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Inhibin alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Inhibin alpha antibody is available for research use only.

Storage Conditions

Aliquot the Inhibin alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Inhibin alpha antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm (secreted)

Image Legend

Western blot testing of human testis tissue lysate with Inhibin alpha antibody. Predicted molecular weight ~40 kDa but may be observed at higher molecular weights due to glycosylation.