Villin Antibody / VIL1

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P09327

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 600-700) from the human protein was used as the immunogen for the Villin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

VIL1/7378R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the Villin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Villin antibody is available for research use only.

Storage Conditions

Aliquot the Villin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Villin antibody should be determined by the researcher.

Location

Cytoplasm, Cell Surface

Image Legend

IHC staining of FFPE human colon adenocarcinoma with Villin antibody (clone VIL1/7378R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.