Villin Antibody / VIL1
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P09327
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 600-700) from the human protein was used as the immunogen for the Villin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
VIL1/7378R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Villin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Villin antibody is available for research use only.
Storage Conditions
Aliquot the Villin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Villin antibody should be determined by the researcher.
Location
Cytoplasm, Cell Surface
Image Legend
Frequently Asked Questions
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