SMARCAL1 Antibody

SmarcAL1 (SWI/SNF-related matrix-associated Actin-dependent regulator of chromatin subfamily A-like protein 1), also known as HARP (hepA-related protein) or HHARP, is a 954 amino acid member of the SWI/SNF family of helicase and ATPase proteins. Localized to the nucleus, SmarcAL1 is a ubiquitously expressed protein that functions in ATP-dependent nucleosomeremodeling activities. SmarcAL1 contains one conserved C-terminal SNF2 domain, one helicase ATP-binding domain and two HARP domains. Defects in the gene encoding SmarcAL1 are the cause of Schimke immuno-osseous dysplasia (SIOD), an autosomal recessive disorder characterized by renal dysfunction, spondyloepiphyseal dysplasia and T cell immunodeficiency.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q9NZC9

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human SMARCAL1 protein was used as the immunogen for the SMARCAL1 antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

PCRP-SMARCAL1-1C4

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SMARCAL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SMARCAL1 antibody is available for research use only.

Storage Conditions

Aliquot the SMARCAL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SMARCAL1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human kidney carcinoma tissue with SMARCAL1 antibody (clone PCRP-SMARCAL1-1C4) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.