Cytotoxic T-lymphocyte protein 4 Antibody / CTLA4 / CD152

Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a member of the CD28 superfamily and is a negative regulator of T cell-mediated immune responses. CTLA-4 expression is induced on the surface of T cells after CD28 binding and activation, and is constitutively expressed on T-regulatory cells, acting as an immune checkpoint inhibitor, downregulating T cell activity. CTLA-4 primarily inactivates T-cell activity by competing with the CD28 costimulatory molecule. CD28 and CTLA-4 share the identical ligands of CD80 and CD86 on antigenpresenting cells; and thus CTLA-4 competes with CD28 function in T-cell survival, proliferation, and recruitment. In particular, CTLA-4 down-modulates CD4+ helper T-cell activity and enhances Treg immunosuppressive functions. CTLA-4 has been shown to play a role in human diseases. CTLA-4 acts as a physiological brake on the activated immune system in order to maintain immune homeostasis. Several suppressive mechanisms for T-cell functions have been attributed to CTLA-4.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P16410

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment of human protein was used as the immunogen for the Cytotoxic T-lymphocyte protein 4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CTLA4/6867R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Cytotoxic T-lymphocyte protein 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Cytotoxic T-lymphocyte protein 4 antibody is available for research use only.

Storage Conditions

Aliquot the Cytotoxic T-lymphocyte protein 4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Cytotoxic T-lymphocyte protein 4 antibody should be determined by the researcher.

Location

Cell surface, Secreted

Image Legend

IHC staining of FFPE human tonsil tissue with Cytotoxic T-lymphocyte protein 4 antibody (clone CTLA4/6867R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.