EpCAM Antibody / Epithelial Cell Adhesion Molecule

Epitope of this mAb is mapped between aa 202-212 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P16422

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human EpCAM fragment from extracellular domain (within amino acids 100-224) was used as the immunogen for the EpCAM antibody. The epitope of this mAb is mapped between amino acids 202-212.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

EGP40/8191R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EpCAM antibody is available for research use only.

Storage Conditions

Aliquot the EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the EpCAM antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human prostate carcinoma tissue with EpCAM antibody (clone EGP40/8191R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.